Resources
Protocols
These Center for Biological Imaging protocols are for general reference ONLY. Please contact a CBI staff member with any questions about your specific experiment — they can provide a protocol and training specific to your project.
Protocols
Cells Immunofluorescence protocol
Immunofluorescence staining protocol for Cells
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Tissue Immunofluorescence protocol
Immunofluorescence staining protocol for Tissue
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Tissue Standard TEM Protocol
Standard EPON embedded TEM protocol for a vriety of tissues.
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Cells Standard TEM Protocol
Standard EPON embedded TEM protocol for Monolayer cells.
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Standard Tissue/Cells SEM protocol
Standard SEM protocol for both tissue and cells.
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LR White TEM protocol
LR White TEM embedding process for gold labeling.
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Immunoelectron TEM protocol
Cryo-sectioning and Immunoelecrton preparation for both tissue and cells.
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General Clearing Protocols
A collection of Clearing protocols we use for deep tissue imaging
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Cytospin Protocol
Protocol for spinning cells onto a slide
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Saponin Protocol
Saponin Immuno Protocol
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Perfusion of Tissue Fixation Protocol
Protocol for tissue perfusiion fixation
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Tissue Fixation and Freezing for IF
Protocol for IF Tissue fixation and freezing
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Recipes
PFA Stock Recipe (8%)
Recipe for mixing up PFA fixitive stock solution
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Gelvatol Recipe
Recipe for mixing up your own Gelvitol mounting media
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Hoechst Recipe (DAPI)
Recipe for mixing up Hoechst Nuclear Stain (DAPI)
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