University of Pittsburgh · Center for Biologic Imaging
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Core Equipment

An installed base of 41 instruments across three access levels — from widefield and confocal systems to multiphoton, super-resolution and electron microscopy.

Nikon STORM
Core · 24/7

Nikon STORM

STORM super resolution confocal.
Room S216.4 Core Instrument training available.
Nikon NI-E II
Core · 24/7

Nikon NI-E II

Upright widefield system capable of imaging 4-color fluorescence and white light with a motorized stage.
Room S216.7 Core Instrument training available.
Nikon NI-E I
Core · 24/7

Nikon NI-E I

Upright widefield system capable of imaging 4-color fluorescence and white light with a motorized stage.
Room S216.7 Core Instrument training available.
Fritz Slide Scanner
Core · 24/7

Fritz Slide Scanner

Slide scanner scope capable of scanning bright-field images very quickly and easily.
Room s216 Core Instrument training available.
Olympus Provis 1 Fluorescence
Core · 24/7

Olympus Provis 1 Fluorescence

Upright widefield capable of imaging white light, 4-color fluorescence, DIC, and polarized light
Room S216.8 Core Instrument training available.
Olympus Fluoview 1000-2 Confocal
Core · 24/7

Olympus Fluoview 1000-2 Confocal

Upright 4-color confocal and DIC system
Room S216.1 Core Instrument training available.
Olympus Fluoview 3000 Confocal
Core · 24/7

Olympus Fluoview 3000 Confocal

Inverted 4-color confocal.
Room S216.9 Core Instrument training available.
Nikon A1 Confocal
Core · 24/7

Nikon A1 Confocal

Inverted 4-color confocal and DIC system with motorized stage.
Room S216.3 Core Instrument training available.
Nikon A1 Confocal
Core · 24/7

Nikon A1 Confocal

Inverted 4-color confocal and DIC system with motorized stage.
Room S216.6 Core Instrument training available.
Analysis Computers
Core · 24/7

Analysis Computers

Multiple analysis computers are available for collaborator use.
Room S216 & CBI lobby Core Instrument training available.
Cryostat
Core · 24/7

Cryostat

Multiple Cryostats are available for frozen slide sections.
Room S233 Core Instrument training available.
Nikon A1 Confocal
Core · 24/7

Nikon A1 Confocal

Standard A1 Confocal
Room S216.2 Core Instrument training available.
Leica High Pressure Freezer
Limited access

Leica High Pressure Freezer

Freeze unfixed samples down for Freeze sub processing
Room S224
Nikon Multiphoton
Limited access

Nikon Multiphoton

Our newest Nikon Multiphoton.
Room S209
Nikon Multiphoton
Limited access

Nikon Multiphoton

Room S218B
Nikon Multiphoton
Limited access

Nikon Multiphoton

Room S208.1
Nikon SIM
Limited access

Nikon SIM

SIM super-resolution confocal.
Room S216.5
Leica STED Super Resolution Confocal
Limited access

Leica STED Super Resolution Confocal

Room S229
Nikon A1R Spectral Confocal
Limited access

Nikon A1R Spectral Confocal

Inverted 4-color confocal and DIC system with motorized stage, with capacity for advanced live cell imaging and spectral detection.
Room S216.8
Leica Freeze Substitution
Limited access

Leica Freeze Substitution

Freeze-sub used for processing TEM samples.
Room S224
Sputter and Carbon Coater
Limited access

Sputter and Carbon Coater

Gold-palladium and carbon coater is available for SEM preparation
Room S236
Critical Point Dryer
Limited access

Critical Point Dryer

Critical point dryer for SEM preparation.
Room S236
Leica Ultramicrotomes
Limited access

Leica Ultramicrotomes

We have multiple Leica Ultramicrotomes
Room S224
Nikon Ti Live Cell
Limited access

Nikon Ti Live Cell

Room 218E
Nikon Eclipse Ti Live Cell
Limited access

Nikon Eclipse Ti Live Cell

Room S230
JEOL 1400-PLUS 120kv TEM
Limited access

JEOL 1400-PLUS 120kv TEM

The 1400-Plus is our 120KV TEM that is available for outside users after training.
Room S226
MesoSPIM
CBI only

MesoSPIM

Room S206
3i Lattice Lightsheet
CBI only

3i Lattice Lightsheet

Room S218C
Jeol Freeze Fracture
CBI only

Jeol Freeze Fracture

Room S235
Olympus Slideview
CBI only

Olympus Slideview

High throughput slide scanner
Room S233
JEOL 1400-FLASH 120KV TEM
CBI only

JEOL 1400-FLASH 120KV TEM

The JEOL 1400-Flash is the newest addition to the EM lab.
Room S227
Nikon A1 Confocal
CBI only

Nikon A1 Confocal

Main lab Nikon A1
Room S232
Nikon A1 Confocal
CBI only

Nikon A1 Confocal

Room S218D
Leica Cryo-ultramicrotome
CBI only

Leica Cryo-ultramicrotome

Leica Ultramicrome with a cryo-kit
Room S224
Caliber Ribbon Scanning Confocal
CBI only

Caliber Ribbon Scanning Confocal

Room S228
Caliber Ribbon Scanning Confocal
CBI only

Caliber Ribbon Scanning Confocal

Room S228
Caliber Ribbon Scanning Confocal
CBI only

Caliber Ribbon Scanning Confocal

Room S228
Nikon Eclipse Ti Live Cell
CBI only

Nikon Eclipse Ti Live Cell

Room S231
Incucyte Incubator Confocal
CBI only

Incucyte Incubator Confocal

Room S235
Nikon Slide Express 2
CBI only

Nikon Slide Express 2

Room S233
JSM-IT710HRLV SEM
CBI only

JSM-IT710HRLV SEM

The JSM-IT710 is our CLEM and large area scanning SEM.
Room S237