TEM TISSUE RUN UP FOR LR WHITE RESIN

Gloves must be worn throughout the procedure. All work should be done in a fume hood.

  1. Fix tissue in 4% paraformaldehyde, and 0.1% glutaraldehyde in PBS (pH 7.4) for 4-6 hours
  2. Wash in 3 changes 0.1 M PBS for 15 minutes each.
  3. Dehydrate in graded series of alcohol (in PBS) for 15 minutes each:
    1. 30% ethanol
    2. 50% ethanol
    3. 70% ethanol
    4. 90% ethanol
    5. 100% ethanol x 3
  4. Infiltrate with LR White resin in 2 one hour changes.
  5. Embed in LR White resin.