Enzo Roche Tunel:for Detection of Apoptotic Cells

Wash cells or Tissue with 1X PBS appx. three times. Put on TUNEL mixture (10 microliters per section), and incubate in humid chamber at 37 degrees C for 60 min. TUNEL mixture: (vortex with each addition) 45 microliters dH2O Buffer 32 microliters CoCL2 20 microliters TdT 1 microliters Biotin 2 microliter Remove slides from incubator and wash off TUNEL mixture thoroughly with 1X PBS Put on streptavidin secondary (488 or Cy3) for 45 minutes to 1hr. Wash thoroughly with 1X PBS Use Hoescht dye for 30 sec. And wash once with 1xPBS Coverslip with gelvatol