8% Paraformaldehyde Solution

  1. For each 100mls of Paraformaldehyde.
  2. Add 8 grams of paraformaldehyde resin to 70 ml of distilled water.
  3. Heat to 60 Celsius(no higher).
  4. Add 1M or 1N NaOH till the solution clears, usually a couple of drops.
  5. Cool to room temperature.
  6. Add 9mls of 10x PBS or 30 mls of 0.3M PBS
  7. PH to 7.4
  8. Filter.
  9. Store at 4 degrees Celsius.