Hoechst (aka ‘bisbenzamide’)

  1. Dissolve Hoechst in water at 1 mg/100mL. Use the Hoescht filter to view cellular nuclei.
  2. Store solution in the dark at 4ºC.
  3. Apply Hoechst to tissue or cell for 30 sec.

Note:Hoechst powder should be stored at –20ºC.
Caution! This substance is carcinogenic and toxic to skin!!


Ordering Information for Bisbenzamide::

Sigma-Aldrich Co.
(2’-[4-Hydroxyphenyl]-5-[4-methyl-1-piperazinyl]-2,5’-bi-1H-benzimidazole) 100 mg